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Ranchers.net

Neurotoxicology. 2006 Feb 13;

Free radical generation of protease-resistant prion after substitution of manganese for copper in bovine brain homogenate.

Deloncle R, Guillard O, Bind JL, Delaval J, Fleury N, Mauco G, Lesage G.

Universite Francois Rabelais de Tours, Bio-Inorganic Chemistry Laboratory, Faculty of Pharmacy, 31 Avenue Monge, 37200 Tours, France.

The exchange between copper and seven transition metals is studied in a bovine brain obex homogenate according to the redox status of the medium. In reductive conditions, almost all the studied metals can substitute for copper when it is in the reduced form Cu(+). This substitution is reversible, since copper uptake as Cu(++) is restored in an oxidizing medium but only Co(++), Ni(++) and Mn(++), in this decreasing order, can substitute perfectly for copper in bovine brain homogenate. To study free radical effects on bovine brain proteins, at first a copper substitution was processed in order to inhibit superoxide dismutase-like protective properties against free radicals in copper metalloproteins. Manganese was selected since a brain copper decrease correlated with a manganese increase is well-known in transmissible spongiform encephalopathies. Results for bovine brain homogenate, initially negative in the Western blot Prionics((R)) test, indicate that the substitution of manganese for copper in a reducing medium and exposure to UVA-induced free radicals produce proteinase K resistant prion. These findings suggest that an impairment in brain metal homeostasis leading to oxidative abnormalities may be involved in transmissible spongiform encephalopathies.

PMID: 16481041

This isn't the newest study I was referring to that supports Mark Purdey's and others like Dr DR Brown - but it is another of many which are stacking up to support the causative relationship between BSE (prion disorders) and improperly balanced metals in the brain.

Free radicals and oxidative damage are responsible for nerve cell death. The self-defence mechanisms are exacerbated by the invasion of the brain by free-radical generating toxins.
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