Research
The identification of disease-induced biomarkers in the urine of BSE infected cattle
Sharon LR Simon , Lise Lamoureux , Margot Plews , Micheal Stobart , Jillian LeMaistre , Ute Ziegler , Catherine Graham , Stefanie Czub , Martin Groschup and J. David Knox
Proteome Science 2008, 6:23doi:10.1186/1477-5956-6-23
Published: 5 September 2008
Abstract (provisional)
Background
The bovine spongiform encephalopathy (BSE) epidemic and the emergence of a new human variant of Creutzfeldt-Jakob Disease (vCJD) have led to profound changes in the production and trade of agricultural goods. The rapid tests currently approved for BSE monitoring in slaughtered cattle are all based on the detection of the disease related isoform of the prion protein, PrPd, in brain tissue and consequently are only suitable for post-mortem diagnosis.
Objectives:
In instances such as assessing the health of breeding stock for export purposes where post-mortem testing is not an option, there is a demand for an ante-mortem test based on a matrix or body fluid that would permit easy access and repeated sampling. Urine and urine based analyses would meet these requirements.
Results
Two dimensional differential gel eletrophoresis (2D-DIGE) and mass spectrometry analyses were used to identify proteins exhibiting differential abundance in the urine of BSE infected cattle and age matched controls over the course of the disease. Multivariate analyses of protein expression data identified a single protein able to discriminate, with 100% accuracy, control from infected samples. In addition, a subset of proteins were able to predict with 85% +/- 13.2 accuracy the time post infection that the samples were collected.
Conclusions These results suggest that in principle it is possible to identify biomarkers in urine useful in the diagnosis, prognosis and monitoring of disease progression of transmissible spongiform encephalopathy diseases (TSEs).
http://www.proteomesci.com/content/6/1/23/abstract
BUT, will the USDA et al let any test be used to detect BSE or any TSE in the USA bovine ???
i doubt it $$$
Sunday, September 07, 2008 CWD LIVE TEST, and the political aspects or fallout of live testing for BSE in cattle in the USA http://chronic-wasting-disease.blogspot.com/2008/09/cwd-live-test-and-political-aspects-or.html
Friday, August 29, 2008
CREEKSTONE VS USDA COURT OF APPEALS, BUSH SAYS, NO WAY, NO HOW
http://madcowtesting.blogspot.com/2008/08/creekstone-vs-usda-court-of-appeals.html
TSS
The identification of disease-induced biomarkers in the urine of BSE infected cattle
Sharon LR Simon , Lise Lamoureux , Margot Plews , Micheal Stobart , Jillian LeMaistre , Ute Ziegler , Catherine Graham , Stefanie Czub , Martin Groschup and J. David Knox
Proteome Science 2008, 6:23doi:10.1186/1477-5956-6-23
Published: 5 September 2008
Abstract (provisional)
Background
The bovine spongiform encephalopathy (BSE) epidemic and the emergence of a new human variant of Creutzfeldt-Jakob Disease (vCJD) have led to profound changes in the production and trade of agricultural goods. The rapid tests currently approved for BSE monitoring in slaughtered cattle are all based on the detection of the disease related isoform of the prion protein, PrPd, in brain tissue and consequently are only suitable for post-mortem diagnosis.
Objectives:
In instances such as assessing the health of breeding stock for export purposes where post-mortem testing is not an option, there is a demand for an ante-mortem test based on a matrix or body fluid that would permit easy access and repeated sampling. Urine and urine based analyses would meet these requirements.
Results
Two dimensional differential gel eletrophoresis (2D-DIGE) and mass spectrometry analyses were used to identify proteins exhibiting differential abundance in the urine of BSE infected cattle and age matched controls over the course of the disease. Multivariate analyses of protein expression data identified a single protein able to discriminate, with 100% accuracy, control from infected samples. In addition, a subset of proteins were able to predict with 85% +/- 13.2 accuracy the time post infection that the samples were collected.
Conclusions These results suggest that in principle it is possible to identify biomarkers in urine useful in the diagnosis, prognosis and monitoring of disease progression of transmissible spongiform encephalopathy diseases (TSEs).
http://www.proteomesci.com/content/6/1/23/abstract
BUT, will the USDA et al let any test be used to detect BSE or any TSE in the USA bovine ???
i doubt it $$$
Sunday, September 07, 2008 CWD LIVE TEST, and the political aspects or fallout of live testing for BSE in cattle in the USA http://chronic-wasting-disease.blogspot.com/2008/09/cwd-live-test-and-political-aspects-or.html
Friday, August 29, 2008
CREEKSTONE VS USDA COURT OF APPEALS, BUSH SAYS, NO WAY, NO HOW
http://madcowtesting.blogspot.com/2008/08/creekstone-vs-usda-court-of-appeals.html
TSS