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Cleansing method to cut CJD risk

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Kathy

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From the USA patent and Trademark office:

United States Patent Application 20040230257
Kind Code A1
Ovokaitys, Todd F. November 18, 2004

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Enhanced bioavailability of nutrients, pharmaceutical agents, and other bioactive substances through laser resonant homogenization or modification of molecular shape or crystalline form


Abstract
A method for improving the bioavailability of a bioactive substance includes subjecting the bioactive substance to laser radiation. The laser radiation modifies the bioactive substance to thereby modify reactions relating thereto in the body. The method enables reductions in inflammation associated with autoimmune diseases, modification of reaction by-products in the body, increased homogenization and flattening of molecular shape and improved methylation. The improved methylation can be utilized to reduce homocysteine blood levels, and to reduce anxiety, depression, paranoia, hostility, somatization (perception of bodily distress) and obsessive-compulsive symptoms. Enhanced nitric oxide generation from modified L-arginine can be used to reduce systolic and diastolic blood pressure, lower total and LDL cholesterol levels, and improve the ratio of total to HDL cholesterol. Increased depth of penetration of sparse constructive nodes of laser radiation may increase the range of photodynamic therapy applications and a wide range of in vitro and in vivo modifications of molecular shape and activity. Laser acoustic resonance can be utilized to increase the homogeneity of crystals, or favor the generation of novel or preferred crystalline forms.

(exerpt taken from patent):

61. The method of reshaping prions or other pathogenic proteins to reduce their pathogenicity according to claim 60, wherein said method comprises selecting a prion or other pathogenic protein to reshape; and determining the peak absorption frequencies of said prions or other pathogenic proteins and their nonpathogenic counterparts using sonoluminescence with CO.sub.2 nucleation absorption spectrum analysis or other spectroscopic method or mathematical modeling; and subjecting said prions or other pathogenic proteins to laser radiation with an amplitude modulation of one or more peak absorption frequencies of normal protein, the pathogenic protein, or the differential absorption pattern between the normal and pathogenic counterpart protein to reshape said prions or other pathogenic proteins to reduce their pathogenicity, and said laser radiation is structured in polarization and wave patterns.
 

Kathy

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Once you have time to absorb what is in this patent application, you will realize what Reader is talking about and also what this patent is suggesting is, that laser irradiation using acoustic sound waves in specific ranges can change the shape of prions.

If it can change it from the "infectious" form back to normal - could it not change it from normal to diseased? You just have to use the right frequencies.

The hypothesis of Mark Purdey suggests that the sonic boom of military planes and the now-grounded Concorde exposed the people and animals to low frequency sound waves that aided in the conversion of the healthy protein to its diseased form.

These sound frequencies definitely excite the metal molecules (ions). If you use a frequency that is too high it will destroy the protein or a prion. Use a frequency at very specific controlled levels, and you may be able to reverse the accumulation of the protein in a specific location within the body. It is very dangerous, of course, as it could cause damage to the healthy tissue nearby, and/or it could create too much debris for the body to eliminate - resulting in a further problem.

It seems that what goes around, eventually comes back around to the work of Mr. Purdey. Yes, I believe a prion could strongly attach to stainless steel surgical instruments - it's magnetized.
 

Kathy

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Do you care to respond to this Reader?

The use of sound frequencies to alter protein conformation?......
The magnetic properties of metals found in prions.... The inability of copper to become magnetized....

I really do want to hear your opinion on these things, honest.
 

Kathy

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I watched a video last evening which showed single cell organisms being destroyed using acoustic frequencies. It was very interesting.

It took quite a bit of time to destroy the organism, from the inside-out. The producer of the show described the scene. The internal matter of the parasite (eg) seemed to turn to mush (self-digest) and all of these tiny little crystals appeared to form. The single cell organisms would swell with fluid from its surrounding, then the internal parts would turn to a mush as well as hundreds of tiny crystal bits. After some time had passed, the cell wall would rupture in a spot, and the internal matter would ooze out.

What intreges me, is the formation of what appeared to be crystalline shapes within the cell.

Dr. T.F. Ovokaity's patent, which I mentioned earlier in this thread, could have real implications in the on-going saga of prion disease.
 

Kathy

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United States Patent Application 20040132109
Kind Code A1
Enari, Masato ; et al. July 8, 2004

--------------------------------------------------------------------------------
Method


Abstract
The invention relates to methods for determining the presence of prions in a tissue/organ or fluid therefrom; said method comprising the steps of: contacting the tissue/organ with one or more devices, wherein said devices are capable of binding prions; removing said devices from contact with said tissue/organ; determining if said devices are binding prions wherein the device is contacted with the tissue/organ for 120 minutes.


--------------------------------------------------------------------------------
Inventors: Enari, Masato; (Chuo-ku, JP) ; Flechsig, Eckhard; (Versbacher, DE) ; Collinge, John; (Queen, GB) ; Weismann, Charles; (London, GB)
Correspondence Name and Address: MARSHALL, GERSTEIN & BORUN LLP
6300 SEARS TOWER
233 S. WACKER DRIVE
CHICAGO
IL
60606
US
(within claims)
8. A method according to any preceding claim wherein the device comprises metal.

9. A method according to claim 8 wherein the metal comprises one or more metal(s) selected from the group consisting of steel, stainless steel, silver, gold or combinations thereof.

10. A method according to claim 8 or claim 9 wherein the device(s) comprise one or more wires.

(within description)
[0012] The device of the present invention may comprise one or more metals or may comprise plastic such as polystyrene, or glass. It is surprisingly disclosed herein that these materials bind prion protein. Preferably, the device of the present invention may comprise one or more metals. Preferably, the metal is any one or more of the metals selected from the group consisting of steel, stainless steel, silver, gold or combinations thereof. More preferably, the metal is stainless steel.
---------------------------------------

The doc who showed prions binding to a wire after touching diseased brains of live mice, have applied for this patent. They will detect the prions by swirling a metal wire against various tissues, then incubating the wire with live nerve cells expressing PrPC, etc.

There research, which they did earlier, did not tell us what happened to the untreated control SS wire which was incubated with nerve cells. The 14 day slide of untreated SS appears to show reactivity (infection) yet the graphic diagram next to it, does not.

Only those cells in direct contact with the SS wire showed "infection". Their words not mine. Direct contact being the main word. Seeding being the next, in mine opinion.


I simply find the application for patents amusing.
 

flounder

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1: J Neurol Neurosurg Psychiatry 1994 Jun;57(6):757-8

Transmission of Creutzfeldt-Jakob disease to a chimpanzee by electrodes contaminated during neurosurgery.

Gibbs CJ Jr, Asher DM, Kobrine A, Amyx HL, Sulima MP, Gajdusek DC.

Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

Stereotactic multicontact electrodes used to probe the cerebral cortex of a middle aged woman with progressive dementia were previously implicated in the accidental transmission of Creutzfeldt-Jakob disease (CJD) to two younger patients. The diagnoses of CJD have been confirmed for all three cases. More than two years after their last use in humans, after three cleanings and repeated sterilisation in ethanol and formaldehyde vapour, the electrodes were implanted in the cortex of a chimpanzee. Eighteen months later the animal became ill with CJD. This finding serves to re-emphasise the potential danger posed by reuse of instruments contaminated with the agents of spongiform encephalopathies, even after scrupulous attempts to clean them.

PMID: 8006664 [PubMed - indexed for MEDLINE]
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8006664&dopt=Abstract

TSS
 

flounder

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New studies on the heat resistance of hamster-adapted scrapie agent: Threshold survival after ashing at 600°C suggests an inorganic template of replication Paul Brown*,dagger , Edward H. RauDagger , Bruce K. Johnson*, Alfred E. Bacote*, Clarence J. Gibbs Jr.*, and D. Carleton Gajdusek§

* Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, and Dagger Environmental Protection Branch, Division of Safety, Office of Research Services, National Institutes of Health, Bethesda, MD 20892; and § Institut Alfred Fessard, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France

Contributed by D. Carleton Gajdusek, December 22, 1999

Abstract

One-gram samples from a pool of crude brain tissue from hamsters infected with the 263K strain of hamster-adapted scrapie agent were placed in covered quartz-glass crucibles and exposed for either 5 or 15 min to dry heat at temperatures ranging from 150°C to 1,000°C. Residual infectivity in the treated samples was assayed by the intracerebral inoculation of dilution series into healthy weanling hamsters, which were observed for 10 months; disease transmissions were verified by Western blot testing for proteinase-resistant protein in brains from clinically positive hamsters. Unheated control tissue contained 9.9 log10LD50/g tissue; after exposure to 150°C, titers equaled or exceeded 6 log10LD50/g, and after exposure to 300°C, titers equaled or exceeded 4 log10LD50/g. Exposure to 600°C completely ashed the brain samples, which, when reconstituted with saline to their original weights, transmitted disease to 5 of 35 inoculated hamsters. No transmissions occurred after exposure to 1,000°C. These results suggest that an inorganic molecular template with a decomposition point near 600°C is capable of nucleating the biological replication of the scrapie agent.

transmissible spongiform encephalopathy | scrapie | prion | medical waste | incineration

Introduction

The infectious agents responsible for transmissible spongiform encephalopathy (TSE) are notoriously resistant to most physical and chemical methods used for inactivating pathogens, including heat. It has long been recognized, for example, that boiling is ineffective and that higher temperatures are most efficient when combined with steam under pressure (i.e., autoclaving). As a means of decontamination, dry heat is used only at the extremely high temperatures achieved during incineration, usually in excess of 600°C. It has been assumed, without proof, that incineration totally inactivates the agents of TSE, whether of human or animal origin. It also has been assumed that the replication of these agents is a strictly biological process (1), although the notion of a "virus" nucleant of an inorganic molecular cast of the infectious beta -pleated peptide also has been advanced (2). In this paper, we address these issues by means of dry heat inactivation studies.

see full text:

http://www.pnas.org/cgi/content/full/97/7/3418
 

flounder

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J Hosp Infect 2002 Jul;51(3):233-5 Related Articles, Links [Click here to read] Contaminated dental instruments.

Smith A, Dickson M, Aitken J, Bagg J.

Infection Research Group, Glasgow Dental Hospital & School, 378 Sauchiehall Street, Glasgow, UK. [email protected]

There is current concern in the UK over the possible transmission of prions via contaminated surgical instruments. Some dental instruments (endodontic files) raise particular concerns by virtue of their intimate contact with terminal branches of the trigeminal nerve. A visual assessment using a dissecting light microscope and scanning electron microscopy of endodontic files after clinical use and subsequent decontamination was performed. The instruments examined were collected from general dental practices and from a dental hospital. Seventy-six per cent (22/29) of the files retrieved from general dental practices remained visibly contaminated, compared with 14% (5/37) from the dental hospital. Current methods for decontaminating endodontic instruments used in dentistry may be of an insufficient standard to completely remove biological material. Improved cleaning methods and the feasibility of single use endodontic instruments require further investigation.

PMID: 12144804 [PubMed - indexed for MEDLINE]

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12144804&dopt=Abstract

J Gen Virol 1999 Nov;80 ( Pt 11):3043-7

Transmission of the 263K scrapie strain by the dental route.

Ingrosso L, Pisani F, Pocchiari M

Laboratory of Virology, lstituto Superiore di Sanita, Viale Regina Elena 299, 00161 Rome, Italy.

Apart from a few cases of iatrogenic and familial human transmissible spongiform encephalopathies (TSEs) or prion diseases, the cause of Creutzfeldt-Jakob disease (CJD) remains unknown. In this paper we investigated the possibility that dental procedures may represent a potential route of infection. This was assessed by using the experimental model of scrapie in hamster. In the first part of this study we found that after intraperitoneal inoculation, oral tissues commonly involved in dental procedures (gingival and pulp tissues) bore a substantial level of infectivity. We also found high scrapie infectivity in the trigeminal ganglia, suggesting that the scrapie agent had reached the oral tissues through the sensitive terminal endings of the trigeminal nerves. In the second part of the study we inoculated a group of hamsters in the tooth pulp and showed that all of them developed scrapie disease. In these animals, we detected both infectivity and the pathological prion protein (PrPsc) in the trigeminal ganglion homolateral to the site of injection but not in the controlateral one. This finding suggests that the scrapie agent, and likely other TSE agents as well, spreads from the buccal tissues to the central nervous system through trigeminal nerves. Although these findings may not apply to humans affected by TSEs, they do raise concerns about the possible risk of transmitting these disorders through dental procedures. Particular consideration should be taken in regard to new variant CJD patients because they may harbour more infectivity in peripheral tissues than sporadic CJD patients.

PMID: 10580068

http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10580068&dopt=Abstract

TSS
 

Kathy

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Exposure to 600°C completely ashed the brain samples, which, when reconstituted with saline to their original weights, transmitted disease to 5 of 35 inoculated hamsters. No transmissions occurred after exposure to 1,000°C.

The samples here were "completely ashed" no protein would remain. Therefore no prion, no virus. Yet 5 of 35 developed disease.


The way magnatism works is this: magnatism is all based on the simple principle of electrons and there behavior. Electrons move around the atom in a specific path. As they do this they are also rotating on there own axis. This movement causes an attraction or repultion from the electrons that are unpaird. They are moving in two directions though causing a negative and positive charge. In the case of magnatism though we find that these elements have a lot of unpaired electrons, in the case of iron, Fe, there are four. What happens then in the case of a natural magnet the unpaired electrons line up or the magnet in a specific
mannor. That is all the atoms with unpaired electrons moving in a direction which causes a certain charge are lined up on one side and all the atoms with the opposite charge move to the other side. The atoms then start to cancel each other out as they approach the center of the magnet. This all happens at the currie point where these atoms are free to move and then when cooled and the metal becomes solid the atoms can
no longer move (barely) causing a "permanent" magnet (as in the diagram on the next page). This same principle can be applied to a piece of metal that has been sitting next to a magnatized piece of metal in that over the long time they are togather the very slow moving atoms in the metal situate in the same fassion also creating a magnet.


After temperatures of 1000 degrees celcius, no transmission occurred.

600 C = 1112 F, 1000 C = 1832 F

Melting points of some metals:
tungsten 3410 C
molybdenum 2623 C
platinum 1772 C
titanium 1660 C
iron 1535 C
colbalt 1495 C
nickle 1455 C
silicon 1410 C
manganese 1246 C
uranium 1132 C
copper 1083 C
gold 1064 C
silver 962 C
strontium 769 C
barium 725 C
aluminum 660 C
magnesium 650 C
zinc 419 C
lead 327 C
cadmium 320 C

Melting point is of interest, as is the currie points of metals, and metal alloys such as Alnico - aluminum-nickel-cobalt, neodymium -iron-boron, Sumarium Cobalt. Since nothing is left after these high temperatures but ash, the answer to factor X must lie with the basic molecular components carbons, nitrogen and metals(minerals).
 

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