Recombinant full-length and deleted PrP was refolded as described with other divalent cations including calcium, magnesium, manganese, nickel, iron and zinc at concentrations of 5 mM.......
In order to determine whether manganese and nickel could replace copper when in competition, full-length PrP was refolded with either nickel and copper or manganese and copper in equimolar concentrations (5 mM). In these cases (Table II) copper was preferentially bound in place of nickel but manganese was bound equivalently with copper (i.e. two atoms each per molecule). When the concentration of manganese was decreased during refolding (5 mM copper, 0.5 mM manganese) the amount of manganese binding was reduced to one-tenth that of copper. These results suggest that manganese can substitute equivalently for copper in the holo-form of PrP.