• If you are having problems logging in please use the Contact Us in the lower right hand corner of the forum page for assistance.

Resistance of Bovine Spongiform Encephalopathy (BSE) Prions

flounder

Well-known member
Resistance of Bovine Spongiform Encephalopathy (BSE) Prions to Inactivation

Kurt Giles1,2, David V. Glidden3, Robyn Beckwith1, Rose Seoanes1, David Peretz1,2¤, Stephen J. DeArmond1,4, Stanley B. Prusiner1,2,5*

1 Institute for Neurodegenerative Diseases, University of California San Francisco, San Francisco, California, United States of America, 2 Department of Neurology, University of California San Francisco, San Francisco, California, United States of America, 3 Department of Epidemiology and Biostatistics, University of California San Francisco, San Francisco, California, United States of America, 4 Department of Pathology, University of California San Francisco, San Francisco, California, United States of America, 5 Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America

Abstract

Distinct prion strains often exhibit different incubation periods and patterns of neuropathological lesions. Strain characteristics are generally retained upon intraspecies transmission, but may change on transmission to another species. We investigated the inactivation of two related prions strains: BSE prions from cattle and mouse-passaged BSE prions, termed 301V. Inactivation was manipulated by exposure to sodium dodecyl sulfate (SDS), variations in pH, and different temperatures. Infectivity was measured using transgenic mouse lines that are highly susceptible to either BSE or 301V prions. Bioassays demonstrated that BSE prions are up to 1,000-fold more resistant to inactivation than 301V prions while Western immunoblotting showed that short acidic SDS treatments reduced protease-resistant PrPSc from BSE prions and 301V prions at similar rates. Our findings argue that despite being derived from BSE prions, mouse 301V prions are not necessarily a reliable model for cattle BSE prions. Extending these comparisons to human sporadic Creutzfeldt-Jakob disease and hamster Sc237 prions, we found that BSE prions were 10- and 106-fold more resistant to inactivation, respectively. Our studies contend that any prion inactivation procedures must be validated by bioassay against the prion strain for which they are intended to be used.


http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1000206


snip...



Greetings,

Considering all the above, and the fact that there are over 20 documented strains of the so called typical scrapie, with new and emerging atypical Scrapie i.e. the NOR-98, and not to forget BSE transmission to sheep and goat. CWD in deer and elk, and just how many strains do we have there? cBSE (typical), hBSE, both documented in North America, and the fact several outbreaks of TME in the USA, no l-BSE ? i question no l-BSE in the USA, due to the fact of the total bungling of the USDA's so called enhanced BSE surveillance program, and then the sudden dramatic decrease in the USDA BSE testing right after being forced to come clean on the negative atypical BSE case in Texas due to the Fong effect, and the Alabama atypical BSE case. i would bet my mothers life on the l-BSE being circulating among the other TSE strains in the USA, just undetected for all the obvious reasons. but how many other strains? now mind you, all of the above strains of TSE transmitting to primates, and mind you, the _typical_ scrapie transmitting to the primate by THERE NON-FORCED ORAL CONSUMPTION. oh yes, but don't forget, typical scrapie has never been documented to transmit to humans. r i g h t ! with the new and emerging human TSEs, some showing up right here in the USA, why is it that the human TSE (all age groups) why are they not all reportable in the USA in every state and Internationally? This must be done. the ramifications from proven transmission studies via the medical and surgical arena's are real, they have been real for some time. Blood now a real issue, now a threat. We must act now, let science take it's course, and put the politics and the industry aside, the conflicts of interest are just to great. these studies take too long due to the incubation period, for partisan, industry, political issues to stand in the way. people are dying. this old study, and some newer ones, always bring it home for me. there is much more to this story than the infamous UKBSEnvCJD hamburger eating adolescents only story. ...TSS




J Neurol Neurosurg Psychiatry. 1994 June; 57(6): 757–758. PMCID: PMC1072988

Copyright notice

Transmission of Creutzfeldt-Jakob disease to a chimpanzee by electrodes contaminated during neurosurgery.

C J Gibbs, Jr, D M Asher, A Kobrine, H L Amyx, M P Sulima, and D C Gajdusek Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892


Abstract

Stereotactic multicontact electrodes used to probe the cerebral cortex of a middle aged woman with progressive dementia were previously implicated in the accidental transmission of Creutzfeldt-Jakob disease (CJD) to two younger patients. The diagnoses of CJD have been confirmed for all three cases. More than two years after their last use in humans, after three cleanings and repeated sterilisation in ethanol and formaldehyde vapour, the electrodes were implanted in the cortex of a chimpanzee. Eighteen months later the animal became ill with CJD. This finding serves to re-emphasise the potential danger posed by reuse of instruments contaminated with the agents of spongiform encephalopathies, even after scrupulous attempts to clean them.

FULL TEXT ;

http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1072988&blobtype=pdf



snip...please see full text ;



Sunday, November 16, 2008
Resistance of Bovine Spongiform Encephalopathy (BSE) Prions to Inactivation

http://bse-atypical.blogspot.com/2008/11/resistance-of-bovine-spongiform.html



TSS
 

Latest posts

Top