P02.29
Selective Susceptibility of Porcine species to Prion Strains as Assayed in
Transgenic Mice Expressing Porcine PrP
Herva, ME1; Espinosa, JC1; Andréoletti, O2; Padilla, D1; Alamillo, E1;
Lacroux, C2; Torres, JM1 1CISA-INIA, Spain; 2INRA-ENVT, France
Background: There have been no reports of naturally occurring TSE in porcine species. Nevertheless, pigs are susceptible to BSE infection but showing a strong transmission barrier by parenteral route. Previous work in our group using transgenic mice expressing porcine PrP (PoPrP-Tg001)
showed a strong transmission barrier for BSE by intracerebral route.
Objectives: The main aim of this work was to characterise the susceptibility of porcine species to different prion strains in transgenic mice expressing porcine PrP Tg mice.
Methods: A wide panel of inocula has been used as intracerebral inoculum in PoPrPTg001: BSE, RML, different human and sheep TSEs.
Results: From the different strains assayed, only the BSE prion strain has
been able to be transmitted to mice. Remarkably, BSE after passage in sheep increased its transmission ability in PoPrP-Tg001 mice that is maintained after subpassage.
Discussion: Our results confirm that porcine species shows an strong
transmission barrier to a wide range of prion strains but the risk of infection of BSE after passage in sheep need to be considered.
http://www.prion2007.com/pdf/Prion%20Book%20of%20Abstracts.pdf
tss
Selective Susceptibility of Porcine species to Prion Strains as Assayed in
Transgenic Mice Expressing Porcine PrP
Herva, ME1; Espinosa, JC1; Andréoletti, O2; Padilla, D1; Alamillo, E1;
Lacroux, C2; Torres, JM1 1CISA-INIA, Spain; 2INRA-ENVT, France
Background: There have been no reports of naturally occurring TSE in porcine species. Nevertheless, pigs are susceptible to BSE infection but showing a strong transmission barrier by parenteral route. Previous work in our group using transgenic mice expressing porcine PrP (PoPrP-Tg001)
showed a strong transmission barrier for BSE by intracerebral route.
Objectives: The main aim of this work was to characterise the susceptibility of porcine species to different prion strains in transgenic mice expressing porcine PrP Tg mice.
Methods: A wide panel of inocula has been used as intracerebral inoculum in PoPrPTg001: BSE, RML, different human and sheep TSEs.
Results: From the different strains assayed, only the BSE prion strain has
been able to be transmitted to mice. Remarkably, BSE after passage in sheep increased its transmission ability in PoPrP-Tg001 mice that is maintained after subpassage.
Discussion: Our results confirm that porcine species shows an strong
transmission barrier to a wide range of prion strains but the risk of infection of BSE after passage in sheep need to be considered.
http://www.prion2007.com/pdf/Prion%20Book%20of%20Abstracts.pdf
tss