I have never AIed a cow in my life however I've heat detected thousands. I've heat detected for two large ranching operations in the area one did 3000 heifers a year the other did around 1200. So I feel I can comment on the subject. Yes the mood of the cow and how calm or upset she is has an effect on how conception rate. However there is a case of one cow that was AIed upside down in the chute and she stuck......