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UPTAKE PrPSc via Peyer's Patches Hamsters Oral Exposure Sc

flounder

Well-known member
Subject: UPTAKE PrPSc via Peyer's Patches in Hamsters after Oral Exposure to Scrapie
Date: April 25, 2006 at 7:58 am PST


Short-term Study of the Uptake of PrPSc by the Peyer's Patches in Hamsters after Oral Exposure to Scrapie

A.-L. Bergströma, T.K. Jensena, P.M.H. Heegaarda, H. Cordesa, V.B. Hansena, H. Laursenb and P. Linda

aDepartment of Veterinary Diagnostics, The Danish Institute for Food and Veterinary Research, Bülowsvej 27, 1790 Copenhagen V, Denmark
bNeuropathology Laboratory, Rigshospitalet, 2100 Copenhagen Ø, Denmark

Received 18 March 2005; accepted 11 August 2005. Available online 10 February 2006.



Summary
The disease-associated prion protein (PrPSc) has been detected in the ileal Peyer's patches of lambs as early as one week after oral exposure to scrapie. In hamsters, the earliest reported time of PrPSc detection in the Peyer's patches after oral exposure to scrapie is 69 days post-infection. To evaluate the acute uptake of inoculum and to investigate whether the Peyer's patches constitute the primary site of entry for scrapie after oral exposure, hamsters were each exposed orally to 1 ml of a 10% brain homogenate from hamsters in the terminal stage of infection with the 263 K strain of the scrapie agent. PrPSc was demonstrated in the Peyer's patches only a few days after exposure, i.e., much earlier than previously reported. This study supports the view that the Peyer's patches constitute at least one of the primary entry sites of PrPSc after oral exposure to scrapie.

Keywords: hamster; Peyer's patches; prion disease; scrapie; sheep


http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WHW-4J7H4J0-1&_user=10&_handle=V-WA-A-W-WU-MsSAYWA-UUA-U-AAVYVCWAWD-AAVZUBWEWD-YAEYBYZZY-WU-U&_fmt=summary&_coverDate=04%2F30%2F2006&_rdoc=3&_orig=browse&_srch=%23toc%236861%232006%23998659997%23620564!&_cdi=6861&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=90cd7410493d9654ce2cd9091f52cb51




TSS
 

Kathy

Well-known member
hamsters were each exposed orally to 1 ml of a 10% brain homogenate

drenched brain material that had been treated and what other lab procedures done to it, prior to insertion in their gut.

Where is the analysis of the brain homogenate for metals?
 

flounder

Well-known member
kathy, i am sure that before they do anything in these lab studies on TSE,
the dip there tissue samples in radioactive materials, then dip them in metals, and the final solution is OPs, before going any further :roll: :wink:

why don't you write them and ask them yourself, then get back to us with the asnwer. :wink:


TSS
 

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