''DAMNING TESTIMONY'' ...TSS
Senator Michael Machado from California
''USDA does not know what's going on''.
''USDA is protecting the industry''.
''SHOULD the state of California step in''
Stanley Prusiner
''nobody has ever ask us to comment''
''they don't want us to comment''
''they never ask''
i tried to see Venemon, after Candian cow was discovered with BSE.
went to see lyle. after talking with him... absolute ignorance... then
thought i
should see Venemon... it was clear his entire policy was to get cattle
bonless beef prods
across the border... nothing else mattered...
his aids confirmed this... 5 times i tried to see Venemon, never worked...
eventually met with carl rove the political... he is the one that
arranged meeting
with Venemon... just trying to give you a sense of the distance... healh
public safety...
was never contacted...
yes i believe that prions are bad to eat and you can die from them...END
Dr. Stan bashing Ann Veneman - 3 minutes
http://maddeer.org/video/embedded/08snip.ram
Senator Michael Machado from California
''USDA does not know what's going on''.
''USDA is protecting the industry''.
''SHOULD the state of California step in''
http://maddeer.org/video/embedded/machado.html
Steven DeArmond - Professor of Neuropathology
http://maddeer.org/video/embedded/dearmond.html
OPERATOR: "Next question comes from Dan Goldstein. Your line is open."
REPORTER: "Yeah. Hi. It's Dan Goldstein. Two questions, one for Dr. Clifford and one for the Secretary. Mr. Secretary, first of all, does this somewhat do you think may shake the confidence of the international community, one, in the ability of the Ames Laboratory and, two, also the efficacy of the IHC test?
"And then also for Dr. Clifford, what does this mean in terms of the protocols? Will you now have to go back and perhaps test more animals with Western Blot tests?"
SEC. JOHANNS: "Let me address the question about the Ames Laboratory, and I'm sure the doctor will want to offer a thought also.
"One of the things we are very, very proud of is that Ames laboratory. They do great work there, and again I remind everybody that the IHC test is an internationally accepted test. And that comes from the OIE, and like I said even amongst scientists you would get debate about the test.
http://www.usda.gov/wps/portal/!ut/p/_s.7_0_A/7_0_1OB?contentidonly=true&contentid=2005/06/0207.xml
I had to throw this in before going further, kind like the infamous "Brownie, you're doing a heck of a job" speech.
Stanley Prusiner - Discoverer of Prions
"THE BEEF IN JAPAN IS SAFER THAN USA BEEF"
"UNDER/OVER 30 MONTH RULE IS STUPID ... ARBITRARY NUMBER"
1 TO A MAXIMUM OF 5 sCJD per million in stans push for the spontaneous theory (patent cdi test$).
90% of CJD is spontaneous, without route and source. (i do not agree, he got the prize, i have no Phds).
stan does mention the atypical TSE in Italian cows and almost goes to sCJD being caused by meat, but
his patent could not bring his heart to do that$ this theory is wrong in the sense of 85% of all
sCJD cases are spontaneous. i have asked him to clarify what figures he thinks of the 85%+ of sCJD
he thinks is spontaneous and what % is from one of the many proven routes and sources of TSE,
and stan has failed to reply to that question. also, the spontaneous theory of TSE has not been proven,
in a sense of producing a TSE that is identical to any field TSE documented to date. IN FACT,
the latest science is proving the UK/BSE/nvCJD only theory is being proven to be false.
Stanley Prusiner - Discoverer of Prions
BEEF FROM JAPAN SAFER FROM MAD COW DISEASE THAN USA BEEF
http://maddeer.org/video/embedded/prusiner.html
-------- Original Message --------
Subject: Strain-specified characteristics of mouse synthetic prions
Date: Tue, 8 Feb 2005 12:58:36 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: [email protected]
##################### Bovine Spongiform Encephalopathy #####################
Published online before print January 25, 2005, 10.1073/pnas.0409079102
PNAS | February 8, 2005 | vol. 102 | no. 6 | 2168-2173
NEUROSCIENCE
Strain-specified characteristics of mouse synthetic prions
Giuseppe Legname *, {dagger} {ddagger} , Hoang-Oanh B. Nguyen *, Ilia
V. Baskakov * § , Fred E. Cohen *, ¶, ||, Stephen J.
DeArmond * {ddagger} , ** and Stanley B. Prusiner *, {dagger} {ddagger}
, ||, {dagger} {dagger}
*Institute for Neurodegenerative Diseases and Departments of {dagger}
Neurology, ¶Cellular and Molecular Pharmacology, ||Biochemistry and
Biophysics, and **Pathology, University of California, San Francisco, CA
94143
Contributed by Stanley B. Prusiner, December 6, 2004
Synthetic prions were produced in our laboratory by using recombinant
mouse prion protein (MoPrP) composed of residues 89-230. The first mouse
synthetic prion strain (MoSP1) was inoculated into transgenic (Tg) 9949
mice expressing N-terminally truncated MoPrP({Delta} 23-88) and WT FVB
mice expressing full-length MoPrP. On first and second passage in Tg9949
mice, MoSP1 prions caused disease in 516 ± 27 and 258 ± 25 days,
respectively; numerous, large vacuoles were found in the brainstem and
gray matter of the cerebellum. MoSP1 prions passaged in Tg9949 mice were
inoculated into FVB mice; on first and second passage, the FVB mice
exhibited incubation times of 154 ± 4 and 130 ± 3 days, respectively. In
FVB mice, vacuolation was less intense but more widely distributed, with
numerous lesions in the hippocampus and cerebellar white matter. This
constellation of widespread neuropatho-logic changes was similar to that
found in FVB mice inoculated with Rocky Mountain Laboratory (RML)
prions, a strain derived from a sheep with scrapie. Conformational
stability studies showed that the half-maximal GdnHCl (Gdn1/2)
concentration for denaturation of MoSP1 prions passaged in Tg9949 mice
was {approx} 4.2 M; passage in FVB mice reduced the Gdn1/2 value to
{approx} 1.7 M. RML prions passaged in either Tg9949 or FVB mice
exhibited Gdn1/2 values of {approx} 1.8 M. The incubation times,
neuropathological lesion profiles, and Gdn1/2 values indicate that MoSP1
prions differ from RML and many other prion strains derived from sheep
with scrapie and cattle with bovine spongiform encephalopathy.
neurodegeneration
------------------------------------------------------------------------
Author contributions: G.L., I.V.B., F.E.C., and S.B.P. designed
research; H.-O.B.N. and S.J.D. performed research; G.L., S.J.D., and
S.B.P. analyzed data; S.B.P. contributed new reagents/analytic tools;
and G.L., S.J.D., and S.B.P. wrote the paper.
Abbreviations: PrP, prion protein; MoPrP, mouse PrP; PrPSc,
disease-causing isoform of PrP; MoSP1, mouse synthetic prion1; rec,
recombinant; Tg, transgenic; RML, Rocky Mountain Laboratory; Gdn1/2,
half-maximal GdnHCl; HuM, human-mouse; PK, proteinase K.
{ddagger} G.L., S.J.D., and S.B.P. have a financial interest in InPro
Biotechnology, Inc.
§ Present address: Medical Biotechnology Center, University of Maryland
Biotechnology Institute, Baltimore, MD 21201.
{dagger} {dagger} To whom correspondence should be addressed. E-mail:
[email protected] .
© 2005 by The National Academy of Sciences
of the USA
http://www.pnas.org/cgi/content/abstract/102/6/2168maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=prion&searchid=1107887303603_6112&stored_search=&FIRSTINDEX=0&volume=102&issue=6&journalcode=pnas
Greetings list members,
> The incubation times, neuropathological lesion profiles, and Gdn1/2
> values indicate that MoSP1 prions differ from RML and many other prion
> strains derived from sheep with scrapie and cattle with bovine
> spongiform encephalopathy.
THIS is what i was most curious about when everyone (well not everybody)
jumped on the ''spontaneous sCJD/TSE bandwagon again''. i wanted to know
if the synthetic TSE (which stan produced) was infectious. HERE we
find it is, BUT, it does not seem to match other TSEs. SO the myth
or theory that 85%+ of all sporadic CJD (or CJDs) just happen
spontaneously (without any route or source of agent) from a misfolding
prion is pretty much put to rest. i never believed it anyway. there was never
any science to back it up. ...TSS
Senator Michael Machado from California
''USDA does not know what's going on''.
''USDA is protecting the industry''.
''SHOULD the state of California step in''
Stanley Prusiner
''nobody has ever ask us to comment''
''they don't want us to comment''
''they never ask''
i tried to see Venemon, after Candian cow was discovered with BSE.
went to see lyle. after talking with him... absolute ignorance... then
thought i
should see Venemon... it was clear his entire policy was to get cattle
bonless beef prods
across the border... nothing else mattered...
his aids confirmed this... 5 times i tried to see Venemon, never worked...
eventually met with carl rove the political... he is the one that
arranged meeting
with Venemon... just trying to give you a sense of the distance... healh
public safety...
was never contacted...
yes i believe that prions are bad to eat and you can die from them...END
Dr. Stan bashing Ann Veneman - 3 minutes
http://maddeer.org/video/embedded/08snip.ram
Senator Michael Machado from California
''USDA does not know what's going on''.
''USDA is protecting the industry''.
''SHOULD the state of California step in''
http://maddeer.org/video/embedded/machado.html
Steven DeArmond - Professor of Neuropathology
http://maddeer.org/video/embedded/dearmond.html
OPERATOR: "Next question comes from Dan Goldstein. Your line is open."
REPORTER: "Yeah. Hi. It's Dan Goldstein. Two questions, one for Dr. Clifford and one for the Secretary. Mr. Secretary, first of all, does this somewhat do you think may shake the confidence of the international community, one, in the ability of the Ames Laboratory and, two, also the efficacy of the IHC test?
"And then also for Dr. Clifford, what does this mean in terms of the protocols? Will you now have to go back and perhaps test more animals with Western Blot tests?"
SEC. JOHANNS: "Let me address the question about the Ames Laboratory, and I'm sure the doctor will want to offer a thought also.
"One of the things we are very, very proud of is that Ames laboratory. They do great work there, and again I remind everybody that the IHC test is an internationally accepted test. And that comes from the OIE, and like I said even amongst scientists you would get debate about the test.
http://www.usda.gov/wps/portal/!ut/p/_s.7_0_A/7_0_1OB?contentidonly=true&contentid=2005/06/0207.xml
I had to throw this in before going further, kind like the infamous "Brownie, you're doing a heck of a job" speech.
Stanley Prusiner - Discoverer of Prions
"THE BEEF IN JAPAN IS SAFER THAN USA BEEF"
"UNDER/OVER 30 MONTH RULE IS STUPID ... ARBITRARY NUMBER"
1 TO A MAXIMUM OF 5 sCJD per million in stans push for the spontaneous theory (patent cdi test$).
90% of CJD is spontaneous, without route and source. (i do not agree, he got the prize, i have no Phds).
stan does mention the atypical TSE in Italian cows and almost goes to sCJD being caused by meat, but
his patent could not bring his heart to do that$ this theory is wrong in the sense of 85% of all
sCJD cases are spontaneous. i have asked him to clarify what figures he thinks of the 85%+ of sCJD
he thinks is spontaneous and what % is from one of the many proven routes and sources of TSE,
and stan has failed to reply to that question. also, the spontaneous theory of TSE has not been proven,
in a sense of producing a TSE that is identical to any field TSE documented to date. IN FACT,
the latest science is proving the UK/BSE/nvCJD only theory is being proven to be false.
Stanley Prusiner - Discoverer of Prions
BEEF FROM JAPAN SAFER FROM MAD COW DISEASE THAN USA BEEF
http://maddeer.org/video/embedded/prusiner.html
-------- Original Message --------
Subject: Strain-specified characteristics of mouse synthetic prions
Date: Tue, 8 Feb 2005 12:58:36 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: [email protected]
##################### Bovine Spongiform Encephalopathy #####################
Published online before print January 25, 2005, 10.1073/pnas.0409079102
PNAS | February 8, 2005 | vol. 102 | no. 6 | 2168-2173
NEUROSCIENCE
Strain-specified characteristics of mouse synthetic prions
Giuseppe Legname *, {dagger} {ddagger} , Hoang-Oanh B. Nguyen *, Ilia
V. Baskakov * § , Fred E. Cohen *, ¶, ||, Stephen J.
DeArmond * {ddagger} , ** and Stanley B. Prusiner *, {dagger} {ddagger}
, ||, {dagger} {dagger}
*Institute for Neurodegenerative Diseases and Departments of {dagger}
Neurology, ¶Cellular and Molecular Pharmacology, ||Biochemistry and
Biophysics, and **Pathology, University of California, San Francisco, CA
94143
Contributed by Stanley B. Prusiner, December 6, 2004
Synthetic prions were produced in our laboratory by using recombinant
mouse prion protein (MoPrP) composed of residues 89-230. The first mouse
synthetic prion strain (MoSP1) was inoculated into transgenic (Tg) 9949
mice expressing N-terminally truncated MoPrP({Delta} 23-88) and WT FVB
mice expressing full-length MoPrP. On first and second passage in Tg9949
mice, MoSP1 prions caused disease in 516 ± 27 and 258 ± 25 days,
respectively; numerous, large vacuoles were found in the brainstem and
gray matter of the cerebellum. MoSP1 prions passaged in Tg9949 mice were
inoculated into FVB mice; on first and second passage, the FVB mice
exhibited incubation times of 154 ± 4 and 130 ± 3 days, respectively. In
FVB mice, vacuolation was less intense but more widely distributed, with
numerous lesions in the hippocampus and cerebellar white matter. This
constellation of widespread neuropatho-logic changes was similar to that
found in FVB mice inoculated with Rocky Mountain Laboratory (RML)
prions, a strain derived from a sheep with scrapie. Conformational
stability studies showed that the half-maximal GdnHCl (Gdn1/2)
concentration for denaturation of MoSP1 prions passaged in Tg9949 mice
was {approx} 4.2 M; passage in FVB mice reduced the Gdn1/2 value to
{approx} 1.7 M. RML prions passaged in either Tg9949 or FVB mice
exhibited Gdn1/2 values of {approx} 1.8 M. The incubation times,
neuropathological lesion profiles, and Gdn1/2 values indicate that MoSP1
prions differ from RML and many other prion strains derived from sheep
with scrapie and cattle with bovine spongiform encephalopathy.
neurodegeneration
------------------------------------------------------------------------
Author contributions: G.L., I.V.B., F.E.C., and S.B.P. designed
research; H.-O.B.N. and S.J.D. performed research; G.L., S.J.D., and
S.B.P. analyzed data; S.B.P. contributed new reagents/analytic tools;
and G.L., S.J.D., and S.B.P. wrote the paper.
Abbreviations: PrP, prion protein; MoPrP, mouse PrP; PrPSc,
disease-causing isoform of PrP; MoSP1, mouse synthetic prion1; rec,
recombinant; Tg, transgenic; RML, Rocky Mountain Laboratory; Gdn1/2,
half-maximal GdnHCl; HuM, human-mouse; PK, proteinase K.
{ddagger} G.L., S.J.D., and S.B.P. have a financial interest in InPro
Biotechnology, Inc.
§ Present address: Medical Biotechnology Center, University of Maryland
Biotechnology Institute, Baltimore, MD 21201.
{dagger} {dagger} To whom correspondence should be addressed. E-mail:
[email protected] .
© 2005 by The National Academy of Sciences
of the USA
http://www.pnas.org/cgi/content/abstract/102/6/2168maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=prion&searchid=1107887303603_6112&stored_search=&FIRSTINDEX=0&volume=102&issue=6&journalcode=pnas
Greetings list members,
> The incubation times, neuropathological lesion profiles, and Gdn1/2
> values indicate that MoSP1 prions differ from RML and many other prion
> strains derived from sheep with scrapie and cattle with bovine
> spongiform encephalopathy.
THIS is what i was most curious about when everyone (well not everybody)
jumped on the ''spontaneous sCJD/TSE bandwagon again''. i wanted to know
if the synthetic TSE (which stan produced) was infectious. HERE we
find it is, BUT, it does not seem to match other TSEs. SO the myth
or theory that 85%+ of all sporadic CJD (or CJDs) just happen
spontaneously (without any route or source of agent) from a misfolding
prion is pretty much put to rest. i never believed it anyway. there was never
any science to back it up. ...TSS
