Question for BSE tester
- Thread starter Bill
- Start date
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bse-tester
Well-known member
Hi Bill, our test is on the verge of going into validation. The cost of validation for validating the test for BSE ONLY is approximately US$1,000,000.00 We are currently in negotiations for funding that will allow us to conduct full scale validation for not only BSE, but vCJD, CJD, Scrapie, CWD, Alzheimers Disease and Parkinsons Disease. The math is simple - it is brutally expensive and so far, we have put up the money out of our own pockets to bring it along to the validation stage where our protocol has been accepted by the United States National Prion Surveillance Labortaroy in Cleveland which has agreed, based upon our science, to conduct all of the above validations. Each validation takes anywhere from 24 - 36 months and we are obviously hoping to conduct a number of them simultaneously.
As for the accuracy of the test - the same laboratory in Cleveland has already spent almost two years conducting tests on our protocol, at our expense, and have found that it is extremely accurate and can identify the presence of both normal (PrP) and malformed (PrPsc) Prions in as little as one (1) Millilitre of urine. Basically, the lab has advised us that if the disease is in the animal or the human host, our test will identify it as being not only there, but what kind of TSE it actually is. In one blind study of a number of CJD patients for example, the test identified 19 positives and the remaining positive as being something different. When that patient was checked, it was found that the urine sample came from an Alzheimers patient. Of course, you can imgaine our excitement. We have all the necessary tissue and urine samples in place and all we need now is the funding. Once we obtain that, we shall begin the validation for BSE and CJD immediately. We have chosen to do those two due to the overwhelming need for enhanced "Risk Management" in the National Herds for both Canada and the USA, and also for the welfare of all humans who can now take a simply urine test to ascertain whether or not they are carrying the malformed prions and this would allow for an extremely aggressive and early develpoment stage treatment to offset the ravages of CJD and provide them, we hope a normal and long life.
The OIE in Paris has formally agreed to also oversee the data that will result from the validation process for the BSE side and they will assess the accuracy of the protocol and render their decision at the end of the validation.
Contrary to one individual's personal attacks on me on this board and this thread regarding profits and greed, we have already spent millions of bucks to get it this far and when we are able to provide our test to the world, we shall indeed take profit and use it to pay us back all of our research and development costs and then all profits will go into further research and post-doctorate studies of prion disease and other animal and human medical sciences.
Of course Bill, I am sure that you will agree, that all we are hoping to achieve by making some up-front money is to cover our costs, as any company, rancher or beef producer would hope to do. Unfortunately, I am being accused of being something of a "bottom-feeder" for wanting to provide a test to help not only beef producers, but people in general. Oh well, the world is full of those who can only see that which they chose to see.
Thanks Bill for letting me explain it to you. Merry Christmas to you and yours.
Ron.
As for the accuracy of the test - the same laboratory in Cleveland has already spent almost two years conducting tests on our protocol, at our expense, and have found that it is extremely accurate and can identify the presence of both normal (PrP) and malformed (PrPsc) Prions in as little as one (1) Millilitre of urine. Basically, the lab has advised us that if the disease is in the animal or the human host, our test will identify it as being not only there, but what kind of TSE it actually is. In one blind study of a number of CJD patients for example, the test identified 19 positives and the remaining positive as being something different. When that patient was checked, it was found that the urine sample came from an Alzheimers patient. Of course, you can imgaine our excitement. We have all the necessary tissue and urine samples in place and all we need now is the funding. Once we obtain that, we shall begin the validation for BSE and CJD immediately. We have chosen to do those two due to the overwhelming need for enhanced "Risk Management" in the National Herds for both Canada and the USA, and also for the welfare of all humans who can now take a simply urine test to ascertain whether or not they are carrying the malformed prions and this would allow for an extremely aggressive and early develpoment stage treatment to offset the ravages of CJD and provide them, we hope a normal and long life.
The OIE in Paris has formally agreed to also oversee the data that will result from the validation process for the BSE side and they will assess the accuracy of the protocol and render their decision at the end of the validation.
Contrary to one individual's personal attacks on me on this board and this thread regarding profits and greed, we have already spent millions of bucks to get it this far and when we are able to provide our test to the world, we shall indeed take profit and use it to pay us back all of our research and development costs and then all profits will go into further research and post-doctorate studies of prion disease and other animal and human medical sciences.
Of course Bill, I am sure that you will agree, that all we are hoping to achieve by making some up-front money is to cover our costs, as any company, rancher or beef producer would hope to do. Unfortunately, I am being accused of being something of a "bottom-feeder" for wanting to provide a test to help not only beef producers, but people in general. Oh well, the world is full of those who can only see that which they chose to see.
Thanks Bill for letting me explain it to you. Merry Christmas to you and yours.
Ron.
Thanks Ron.
It seemed there were a couple of other outfits that were at the cutting edge of developing live tests over the past 3 years but it has gotten awful quiet so I ws curious as to where your's was at.
Merry Christmas and all the best in '08 to you as well.
It seemed there were a couple of other outfits that were at the cutting edge of developing live tests over the past 3 years but it has gotten awful quiet so I ws curious as to where your's was at.
Merry Christmas and all the best in '08 to you as well.
PORKER
Well-known member
Merry Christmas Ron, Remember that I wished for a BSE free herd someday and it is almost here. Bless You in helping mankind and his animals.
Bill said:
hi bill,
here is some other data on the topic you might be interested in ;
Prion detection by an amyloid seeding assay
David W. Colby*, Qiang Zhang*,, Shuyi Wang*, Darlene Groth*, Giuseppe
Legname*,, Detlev Riesner, and Stanley B. Prusiner*,¶,||
*Institute for Neurodegenerative Diseases and ¶Departments of Neurology and
Biochemistry and Biophysics, University of California, San Francisco, CA
94143; and Institut für Physikalische Biologie, Heinrich-Heine Universität,
40225 Düsseldorf, Germany
Contributed by Stanley B. Prusiner, October 25, 2007 (sent for review
September 18, 2007)
Abstract
Polymerization of recombinant prion protein (recPrP), which was produced in
bacteria, into amyloid fibers was accompanied by the acquisition of prion
infectivity. We report here that partially purified preparations of prions
seed the polymerization of recPrP into amyloid as detected by a fluorescence
shift in the dye Thioflavin T. Our amyloid seeding assay (ASA) detected
PrPSc, the sole component of the prion, in brain samples from humans with
sporadic Creutzfeldt–Jakob disease, as well as in rodents with experimental
prion disease. The ASA detected a variety of prion strains passaged in both
mice and hamsters. The sensitivity of the ASA varied with strain type; for
hamster Sc237 prions, the limit of detection was 1 fg. Some prion strains
consist largely of protease-sensitive PrPSc (sPrPSc), and these strains were
readily detected by ASA. Our studies show that the ASA provides an
alternative methodology for detecting both sPrPSc and protease-resistant
PrPSc that does not rely on protease digestion or immunodetection.
prion protein | PrPSc | Thioflavin T | protease-sensitive | femtogram
----------------------------------------------------------------------------
----
Footnotes
Author contributions: D.W.C., Q.Z., G.L., D.R., and S.B.P. designed
research; D.W.C., Q.Z., S.W., and D.G. performed research; D.W.C. and S.B.P.
analyzed data; and D.W.C. and S.B.P. wrote the paper.
Present address: Buck Institute for Age Research, Novato, CA 94945.
Present address: Neurobiology Sector, Scuola Internazionale Superiore di
Studi Avanzati, Trieste 34012, Italy.
Conflict of interest statement: D.G., G.L., D.R., and S.B.P. have financial
interest in InPro Biotechnology, Inc.
||To whom correspondence should be addressed. E-mail: [email protected]
http://www.pnas.org/cgi/content/abstract/0710152105v1?etoc
ALSO, see potential TSE rapid test data in the pipeline here ;
http://www.prion2007.com/pdf/Prion%20Book%20of%20Abstracts.pdf
EFSA Scientific Report on the evaluation of rapid ante mortem BSE test1
Question number: EFSA-Q-2003-084
Adopted date: 27/11/2006
Summary
The European Food Safety Authority (EFSA) and its Scientific Expert Working Group on Transmissible Spongiform Encephalopathy (TSE) Testing were asked by the European Commission (EC) to take over the mandate of the former Scientific Steering Committee (SSC) for the scientific evaluation of rapid TSE/BSE (Bovine Spongiform Encephalopathy) tests. At present 12 rapid BSE test kits are approved by the EC for the post mortem testing of slaughtered cattle in accordance with the TSE Regulation (EC) No 999/2001. Following an EC call for expression of interest in the Official Journal of the European Union (No C15) on 22 January 2003, several parties indicated their interest in participating in the third European evaluation exercise for newly developed rapid post mortem and live animal TSE/BSE tests.
Expressions of interest were invited from those who had tests in advanced stages of development or available for use for the diagnosis of BSE in live cattle. Applications were received from six companies presenting six different tests. In order to ensure that useful tests would be widely available, applicants were also requested to give assurances that they were prepared to make their tests available on a non discriminatory basis following the evaluation. A panel of external scientists assessed all applications based on the pre-defined criteria, covering e.g. the scientific basis of the test, available experimental evidence, practicality of the sampling and testing procedures and stage of development of the test.
Following this assessment 1 test was selected for the evaluation exercise. This joint application was presented by two companies: Scil Diagnostics GmbH, Martinsried, Germany and DiaSpec GmbH, Freiburg, Germany for the "AquaSpec BSE" rapid ante mortem test. Evaluation was based on the EFSA Scientific Report on the Design of a Field Trial Protocol for the evaluation of BSE Tests for Live Cattle adopted on 1 July 2004. On 10 October 2006, EFSA has received the report of the European Commission's Institute of Reference Materials and Measurements (IRMM) on the evaluation of DiaSpec's rapid ante mortem BSE test (IRMM, 2006).
Based on the overall assessment covering the application dossier and a phase I laboratory evaluation, the experts of EFSA´s Working Group on TSE Testing express their opinion on the evaluated ante mortem BSE test. As the submitted AquaSpec BSE rapid ante mortem BSE Test could not succeed the laboratory trial, the overall assessment is negative.
Therefore, the experts of the EFSA WG on TSE Testing concluded that the AquaSpec BSE ante mortem rapid BSE Test did not meet the predefined criteria and therefore do not recommend the AquaSpec BSE rapid ante mortem test (Scil Diagnostics GmbH, Martinsried, Germany and DiaSpec GmbH, Freiburg, Germany) for approval by the European Commission.
____________________________
1 For citation purposes: Scientific Report of the European Food Safety Authority on Transmissible Spongiform Encephalopathy (TSE) on a request from the European Commission on the evaluation of a rapid ante mortem BSE test, The EFSA Journal (2006) 95, 1-14
Publication date: 19/12/2006
Last updated: 19/12/2006
Summary
http://www.efsa.europa.eu/EFSA/Scientific_Document/biohaz_sr_ej95_live_animal_bse_test_summary_en.pdf
Document
http://www.efsa.europa.eu/EFSA/Scientific_Document/biohaz_sr_ej95_live_animal_bse_test_en.pdf
USDA CERTIFIED BSE TESTING BLUNDERS
http://madcowtesting.blogspot.com/
TSS
bse-tester
Well-known member
Further to your question Bill regarding those other tests that have apparently fallen between the cracks. Some are still ongoing and the majority, if not all of them were involved in the detection of PrPsc in blood.
Even though we initially looked at developing a "live test" by using blood as the sample from the host, we very quickly changed our minds due to the extremely comlex matrix that blood is. Blood is absolutely full of many many proteins and other agents that will, without any doubt whatsoever, create false positives virtually with every test. So it becomes a consumate waste of time to try to identfy the necessary agent (PrPsc) within a matrix that is actually doing its best to fool you each and every time.
This is why so many companies have attempted it, announced their initial findings, then faded away as they soon realized that blood is a very complex liquid to use.
We found that urine is by far, the best agent to use in that it is already representative of what was in the blood, has been filtered naturally by the kidney's and is easily accessable without too much invasive techniques which the animal or the human will have to be subjected to. Urine is also easier to use in the lab inthat it can be concentrated faster without having to go through a lengthy and costly seperation process whereby the blood is broken down through many procedures to illiminate all of the other "stuff" that is not part of the final process.
We do know that our protocol will work, as it has in the early stages in the lab in Cleveland. What we need to do now is tweek it and with our totally extra-special little antibody that we use to bind to the PrPsc, we then can identify the presence of that little bad guy in urine within 24 hours or less.
Essentially, if you can imagine that you are going to ship your animals to the slaughterhouse say Friday, then you can arrange to have the local vet come to your farm on Tuesday prior and take the samples from each animal that is going to the plant. By isolating those animals and then receiving the complete and total all clear from the lab, you can now ship animals that are 100% clear of BSE. Now that makes your product somewhat a little more desirable than others in that it has now been scientifically tested and deemed to be free of BSE. I am sure you can see the potential for the enhancement of not only your "Risk Managment" but als oyour market and also the price you can expect to receive for those animals may well increase as well. Within a few yews, BSE will become a thing of the past in the sense that any animla found to have the disease will never be shipped to the slaughterhouse at all. Taking that animal out of the loop will make beef producers free to sell their product at the kind of prices they once enjoyed in my opinion and without having to worry whether or not one of their animals will be rejected.
Of course, that animal, once it enters the kill floor is subjected to another test which will act as a confirmatory test to be kept on file, along with the tissues which will be kept in a minus 80C freezer for the expected life of the product from kill to consumption.
Merry Christmas and a very Happy New Year folks.
Ron.
Even though we initially looked at developing a "live test" by using blood as the sample from the host, we very quickly changed our minds due to the extremely comlex matrix that blood is. Blood is absolutely full of many many proteins and other agents that will, without any doubt whatsoever, create false positives virtually with every test. So it becomes a consumate waste of time to try to identfy the necessary agent (PrPsc) within a matrix that is actually doing its best to fool you each and every time.
This is why so many companies have attempted it, announced their initial findings, then faded away as they soon realized that blood is a very complex liquid to use.
We found that urine is by far, the best agent to use in that it is already representative of what was in the blood, has been filtered naturally by the kidney's and is easily accessable without too much invasive techniques which the animal or the human will have to be subjected to. Urine is also easier to use in the lab inthat it can be concentrated faster without having to go through a lengthy and costly seperation process whereby the blood is broken down through many procedures to illiminate all of the other "stuff" that is not part of the final process.
We do know that our protocol will work, as it has in the early stages in the lab in Cleveland. What we need to do now is tweek it and with our totally extra-special little antibody that we use to bind to the PrPsc, we then can identify the presence of that little bad guy in urine within 24 hours or less.
Essentially, if you can imagine that you are going to ship your animals to the slaughterhouse say Friday, then you can arrange to have the local vet come to your farm on Tuesday prior and take the samples from each animal that is going to the plant. By isolating those animals and then receiving the complete and total all clear from the lab, you can now ship animals that are 100% clear of BSE. Now that makes your product somewhat a little more desirable than others in that it has now been scientifically tested and deemed to be free of BSE. I am sure you can see the potential for the enhancement of not only your "Risk Managment" but als oyour market and also the price you can expect to receive for those animals may well increase as well. Within a few yews, BSE will become a thing of the past in the sense that any animla found to have the disease will never be shipped to the slaughterhouse at all. Taking that animal out of the loop will make beef producers free to sell their product at the kind of prices they once enjoyed in my opinion and without having to worry whether or not one of their animals will be rejected.
Of course, that animal, once it enters the kill floor is subjected to another test which will act as a confirmatory test to be kept on file, along with the tissues which will be kept in a minus 80C freezer for the expected life of the product from kill to consumption.
Merry Christmas and a very Happy New Year folks.
Ron.
TimH
Well-known member
bse-tester said:
Subjected to ANOTHER test????
Who pays for that one Ron??? Then why even bother with your's ???? :lol: :lol: :lol: :lol: :lol: :lol: :lol: :lol:You really should try selling used cars,life insurance or home barber kits.
:lol: :lol: :lol: :lol: :lol:
bse-tester
Well-known member
The idiot TimH wrote:
It is part of the same test you ignorant pathetic little moron. All tests, whether post or ante-mortem typically require a follow-up test for confirmatory purposes. But that concept like most other simple things, seem to be too much for you to grasp.
You have gone way too far with your personal attacks on me and frankly, I am sick and tired of havng to tolerate them any further. :roll: :roll:
It is part of the same test you ignorant pathetic little moron. All tests, whether post or ante-mortem typically require a follow-up test for confirmatory purposes. But that concept like most other simple things, seem to be too much for you to grasp.
You have gone way too far with your personal attacks on me and frankly, I am sick and tired of havng to tolerate them any further. :roll: :roll:
A Potential Blood Test for Transmissible Spongiform Encephalopathies by Detecting Carbohydrate-Dependent Aggregates of PrPres-Like Proteins in Scrapie-Infected Hamster Plasma
Kazuo Tsukui1), Masuhiro Takata2) and Kenji Tadokoro1)
1) Central Blood Institute, The Japanese Red Cross Society
2) Research Center for Prion Diseases, National Institute of Animal Health, Japan
(Received: June 13, 2007)
(Accepted for publication: October 17, 2007)
Abstract:
PrPres has rarely been detected in blood (except in leukocytes) even in diseased animal models that are known to contain a large amount of PrPres in infected tissues. It seems likely that PrPres detection in blood is difficult because of the low titer of infectious material within the blood. Here, we demonstrate the detection of proteinase K-resistant 3F4-reactive protein in the plasma of scrapie-infected hamsters but not in the plasma of mock-infected hamsters by partial purification using a novel method termed "acidic SDS precipitation," in conjunction with a highly sensitive chemiluminescence detection system used to show the presence of PrP at a concentration equivalent to 1.4×10-9 g of brain homogenate or 1.5×10-12 g (6.5×10-17 mol) of rPrP by conventional Western blotting. The 3F4-reactive proteins in scrapie-infected hamster plasma often resulted in multiple Mw protein bands occurring at higher Mw positions than the position of the di-glycosyl PrP molecule. Mixing scrapie-infected hamster brain homogenate with mock-infected hamster plasma resulted in the formation of similar Mw positions for multiple 3F4-reactive proteins. Predigestion of carbohydrate side chains from the proteins in the plasma or brain homogenate before mixing resulted in failure to obtain these multiple 3F4-reactive proteins. These observations indicate that PrPres aggregated with other proteins in the plasma through carbohydrate side chains and was successfully detected in the plasma of scrapie-infected hamsters. Counterparts in these aggregates with PrPres-like proteins in scHaPl are not known but any that exist should resist the PK digestion.
Release Date: December 20, 2007
http://www.jstage.jst.go.jp/article/mandi/51/12/51_1221/_article
FULL TEXT ;
http://www.jstage.jst.go.jp/article/mandi/51/12/1221/_pdf
REFERENCES
http://www.jstage.jst.go.jp/article/mandi/51/12/51_1221/_cit
A RE-ASSESSMENT OF THE POTENTIAL RISK OF VCJD TRANSMISSION VIA DENTISTRY
ISSUE
see full text 17 pages ;
http://www.seac.gov.uk/papers/99-7.pdf
full text ;
http://seac992007.blogspot.com/
vCJD transfusion-associated Fourth Case UK
http://vcjdtransfusion.blogspot.com/
vCJD case study highlights blood transfusion risk
http://vcjdblood.blogspot.com/
CREUTZFELDT JAKOB DISEASE MAD COW BASE UPDATE USA
http://cjdmadcowbaseoct2007.blogspot.com/
Monitoring the occurrence of emerging forms of Creutzfeldt-Jakob disease in
the United States
http://cjdusa.blogspot.com/
CJD QUESTIONNAIRE
http://cjdquestionnaire.blogspot.com/
TSS
Kazuo Tsukui1), Masuhiro Takata2) and Kenji Tadokoro1)
1) Central Blood Institute, The Japanese Red Cross Society
2) Research Center for Prion Diseases, National Institute of Animal Health, Japan
(Received: June 13, 2007)
(Accepted for publication: October 17, 2007)
Abstract:
PrPres has rarely been detected in blood (except in leukocytes) even in diseased animal models that are known to contain a large amount of PrPres in infected tissues. It seems likely that PrPres detection in blood is difficult because of the low titer of infectious material within the blood. Here, we demonstrate the detection of proteinase K-resistant 3F4-reactive protein in the plasma of scrapie-infected hamsters but not in the plasma of mock-infected hamsters by partial purification using a novel method termed "acidic SDS precipitation," in conjunction with a highly sensitive chemiluminescence detection system used to show the presence of PrP at a concentration equivalent to 1.4×10-9 g of brain homogenate or 1.5×10-12 g (6.5×10-17 mol) of rPrP by conventional Western blotting. The 3F4-reactive proteins in scrapie-infected hamster plasma often resulted in multiple Mw protein bands occurring at higher Mw positions than the position of the di-glycosyl PrP molecule. Mixing scrapie-infected hamster brain homogenate with mock-infected hamster plasma resulted in the formation of similar Mw positions for multiple 3F4-reactive proteins. Predigestion of carbohydrate side chains from the proteins in the plasma or brain homogenate before mixing resulted in failure to obtain these multiple 3F4-reactive proteins. These observations indicate that PrPres aggregated with other proteins in the plasma through carbohydrate side chains and was successfully detected in the plasma of scrapie-infected hamsters. Counterparts in these aggregates with PrPres-like proteins in scHaPl are not known but any that exist should resist the PK digestion.
Release Date: December 20, 2007
http://www.jstage.jst.go.jp/article/mandi/51/12/51_1221/_article
FULL TEXT ;
http://www.jstage.jst.go.jp/article/mandi/51/12/1221/_pdf
REFERENCES
http://www.jstage.jst.go.jp/article/mandi/51/12/51_1221/_cit
A RE-ASSESSMENT OF THE POTENTIAL RISK OF VCJD TRANSMISSION VIA DENTISTRY
ISSUE
see full text 17 pages ;
http://www.seac.gov.uk/papers/99-7.pdf
full text ;
http://seac992007.blogspot.com/
vCJD transfusion-associated Fourth Case UK
http://vcjdtransfusion.blogspot.com/
vCJD case study highlights blood transfusion risk
http://vcjdblood.blogspot.com/
CREUTZFELDT JAKOB DISEASE MAD COW BASE UPDATE USA
http://cjdmadcowbaseoct2007.blogspot.com/
Monitoring the occurrence of emerging forms of Creutzfeldt-Jakob disease in
the United States
http://cjdusa.blogspot.com/
CJD QUESTIONNAIRE
http://cjdquestionnaire.blogspot.com/
TSS
flounder said:
TimH
Well-known member
bse-tester said:
Either get used to it or DO something about it, Bottom Feeder.
Would you like directions to my house???
What is the point of having a pre-mortem BSE test if you need another post-mortem test to confirm the results???????? :lol: :lol: :lol: :lol:
Merry Christmas Ronnie-Baby!!!!!
bse-tester
Well-known member
bsetester wrote:
TimH wrote as his incredible reply to me:
So, to answer your question which I wonder if you will actually grasp or even try to grasp:
First, you take the initial test to see if there is any evidence of infection. Second, you take a another sample, test it, confirm your first findings, then you store that sample and the results for a period that is equal to the average time it takes for the product (meat) to make it to the consumers kitchen and then be consumed. With me so far Timmy?? :roll: :roll:
This is done to provide a complete and traceable inventory and identification protocol of the actual animal from which that sample was taken. It is called "following the rules of protocol" that are commonly practiced in most laboratories around the world that deal with extremely contagious and dangerous level 3 or 4 type diseases. We are proposing that this protocol is enhanced to include "maintaining a traceable chain of custody from producer to consumer"
Now Timmy, you can read that or have someone else read it to you slowly and absorb it and deal with it as you please. I am finished with you from here on and will not respond to your ignorance or insults any more.
Timh, now you have shown all of us your true colors. You do not give a rat's arse about having any decent debate on this board and you care only to undermine those who chose to share information, much of which you do not understand obviously, and then you spew your insults, mainly directed at or to me. I personally am finished with you and my only regret is that you who cannot see anything positive in life and will always only embrace the negatives which, I see is your only concern.
I am saddened by the fact that you take this position with me because I have done nothing to you to bring this about. Certainly, I have responded to your insults and your rhetoric, but this all began with you and your consumate ignorance. So I shall leave you to your stupidity and your insulting manner and have nothing further to do with you.
Even though you display an incredible propencity towards being relentlessly ignorant and insulting towards me, I will still wish you and yours a very Merry Christmas and hopefully, a very good New Year as I do to all on this board.
TimH wrote as his incredible reply to me:
So, to answer your question which I wonder if you will actually grasp or even try to grasp:
First, you take the initial test to see if there is any evidence of infection. Second, you take a another sample, test it, confirm your first findings, then you store that sample and the results for a period that is equal to the average time it takes for the product (meat) to make it to the consumers kitchen and then be consumed. With me so far Timmy?? :roll: :roll:
This is done to provide a complete and traceable inventory and identification protocol of the actual animal from which that sample was taken. It is called "following the rules of protocol" that are commonly practiced in most laboratories around the world that deal with extremely contagious and dangerous level 3 or 4 type diseases. We are proposing that this protocol is enhanced to include "maintaining a traceable chain of custody from producer to consumer"
Now Timmy, you can read that or have someone else read it to you slowly and absorb it and deal with it as you please. I am finished with you from here on and will not respond to your ignorance or insults any more.
Timh, now you have shown all of us your true colors. You do not give a rat's arse about having any decent debate on this board and you care only to undermine those who chose to share information, much of which you do not understand obviously, and then you spew your insults, mainly directed at or to me. I personally am finished with you and my only regret is that you who cannot see anything positive in life and will always only embrace the negatives which, I see is your only concern.
I am saddened by the fact that you take this position with me because I have done nothing to you to bring this about. Certainly, I have responded to your insults and your rhetoric, but this all began with you and your consumate ignorance. So I shall leave you to your stupidity and your insulting manner and have nothing further to do with you.
Even though you display an incredible propencity towards being relentlessly ignorant and insulting towards me, I will still wish you and yours a very Merry Christmas and hopefully, a very good New Year as I do to all on this board.
TimH
Well-known member
bse-tester said:bsetester wrote:
TimH wrote as his incredible reply to me:
So, to answer your question which I wonder if you will actually grasp or even try to grasp:
First, you take the initial test to see if there is any evidence of infection. Second, you take a another sample, test it, confirm your first findings, then you store that sample and the results for a period that is equal to the average time it takes for the product (meat) to make it to the consumers kitchen and then be consumed. With me so far Timmy?? :roll: :roll:
This is done to provide a complete and traceable inventory and identification protocol of the actual animal from which that sample was taken. It is called "following the rules of protocol" that are commonly practiced in most laboratories around the world that deal with extremely contagious and dangerous level 3 or 4 type diseases. We are proposing that this protocol is enhanced to include "maintaining a traceable chain of custody from producer to consumer"
Now Timmy, you can read that or have someone else read it to you slowly and absorb it and deal with it as you please. I am finished with you from here on and will not respond to your ignorance or insults any more.
Timh, now you have shown all of us your true colors. You do not give a rat's arse about having any decent debate on this board and you care only to undermine those who chose to share information, much of which you do not understand obviously, and then you spew your insults, mainly directed at or to me. I personally am finished with you and my only regret is that you who cannot see anything positive in life and will always only embrace the negatives which, I see is your only concern.
I am saddened by the fact that you take this position with me because I have done nothing to you to bring this about. Certainly, I have responded to your insults and your rhetoric, but this all began with you and your consumate ignorance. So I shall leave you to your stupidity and your insulting manner and have nothing further to do with you.
Even though you display an incredible propencity towards being relentlessly ignorant and insulting towards me, I will still wish you and yours a very Merry Christmas and hopefully, a very good New Year as I do to all on this board.
As usual,bottom feeder rattles on without answering the question.
Same old.
Some old.
Hope you read this reply up and down.
Or, horizontaly and vertically.
Lest you miss something.
End.
bse-tester
Well-known member
MY ANSWER TO THE IDIOT IS AND WAS AS FOLLOWS:
TimH replies with this moronic and epic stupidity:
As usual,bottom feeder rattles on without answering the question.
Same old.
Some old.
Hope you read this reply up and down.
Or, horizontaly and vertically.
Lest you miss something.
End. :roll: :roll: :roll: :roll: :roll: :roll: :roll: :roll: :roll: :roll: :roll:
Someone else can answer this idiot! He has now truly shown all of us how deep his ignorance goes.
How much more of an answer can one hope to get????
rkaiser
Well-known member
At the risk of being attacked as a filthy salesman Tim, I wonder why a test, which came up with a slight amount of evidence of BSE would not be backed up by another.
I personally believe that testing for this "BSEconomic" crap is the best way for producers to leave it behind.
A live animal test would be one less monetary excuse not to test. And that is all that the fight against testing has. Monetary excuse. If you would rather continue to see the multinational packers and those up the line continue to reap all of the profits of BSEconomics Tim, I can not change your mind. However every situation has positive and negative, and while we sit back and complain about the negatives - the packers use the positives to reap rewards. Had we producers chosen the proactive route of testing for marketing purposes from the beginning of this debacle - life would be a whole lot different right now.
I would much rather see Ron put a few bucks back in his pocket, and even set himself up a nice little retirement, than continue to sit and watch post slaughter profits increase and ranchers potential decrease. Especially when the solution is so obvious ----- test the product --- increase demand--- Screw with Cargill and Tysons captive supply.
I personally believe that testing for this "BSEconomic" crap is the best way for producers to leave it behind.
A live animal test would be one less monetary excuse not to test. And that is all that the fight against testing has. Monetary excuse. If you would rather continue to see the multinational packers and those up the line continue to reap all of the profits of BSEconomics Tim, I can not change your mind. However every situation has positive and negative, and while we sit back and complain about the negatives - the packers use the positives to reap rewards. Had we producers chosen the proactive route of testing for marketing purposes from the beginning of this debacle - life would be a whole lot different right now.
I would much rather see Ron put a few bucks back in his pocket, and even set himself up a nice little retirement, than continue to sit and watch post slaughter profits increase and ranchers potential decrease. Especially when the solution is so obvious ----- test the product --- increase demand--- Screw with Cargill and Tysons captive supply.
Sandhusker
Well-known member
Amen. Having a test that would let us know the true extent of the disease would be the best weapon we could have to eradicate BSE, which should be our goal. Should Ron get a few dollars in his pocket, or even filthy rich, it would still be money well spent for all of us.
Timmy may see the light once he conquors puberty and his hormones settle down.
Timmy may see the light once he conquors puberty and his hormones settle down.
bse-tester
Well-known member
Well put guys, but one thing to make note of please.
All I want is to see our research and development costs paid back. Any company will hope for that - that is part of business.
After that, as I have stated time and time again, all profits will go into continued research for prion disease around the world. That is why we got involved in the first place - firstly to develop and validate the test and then to make sure it is used to accomplish two main goals:
Eradication of BSE
Early diagnosis of CJD.
These goals have now come to include early diagnosis of all TSE's, Alzheiner's Disease and Parkinsons disease and the enhancement of "Risk Management" and hopefully, eradication of BSE for those National Herds of countries known to have BSE.
Ron.
All I want is to see our research and development costs paid back. Any company will hope for that - that is part of business.
After that, as I have stated time and time again, all profits will go into continued research for prion disease around the world. That is why we got involved in the first place - firstly to develop and validate the test and then to make sure it is used to accomplish two main goals:
Eradication of BSE
Early diagnosis of CJD.
These goals have now come to include early diagnosis of all TSE's, Alzheiner's Disease and Parkinsons disease and the enhancement of "Risk Management" and hopefully, eradication of BSE for those National Herds of countries known to have BSE.
Ron.
PORKER
Well-known member
These goals have now come to include early diagnosis of all TSE's, Alzheiner's Disease and Parkinsons disease and the enhancement of "Risk Management" and hopefully, eradication of BSE for those National Herds of countries known to have BSE. Also the variants of CJD which I believe could be called dementia .
TimH
Well-known member
Sandhusker said:
Maybe Sandhusker will see the light once he pulls his nose out of bse-tester's butt-crack. Contrary to the spin you girls are trying to put on this, I have no problem with Ron or his test making money. What I do have a problem with, is good old Ronnie-baby implying that my product is unsafe unless it is tested with his product.
That is fear-mongering and that is how a bottom-feeder turns a profit.
Savvy??
Sandhusker
Well-known member
Tim, it isn't Ron saying your product is unsafe unless proven otherwise, that's what the world is saying. That's why borders shut and why there are places Canadian beef can't be sold.
